{"id":125,"date":"2023-11-14T01:26:07","date_gmt":"2023-11-14T01:26:07","guid":{"rendered":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/?page_id=125"},"modified":"2023-11-15T14:49:14","modified_gmt":"2023-11-15T14:49:14","slug":"resources","status":"publish","type":"page","link":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/resources\/","title":{"rendered":"Resources"},"content":{"rendered":"<p><div class=\"et_d4_element et_pb_section et_pb_section_0 et_pb_with_background  et_pb_css_mix_blend_mode et_section_regular et_block_section\" >\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_d4_element et_pb_row et_pb_row_0  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_0  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_0  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h1>Resources<\/h1><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_section et_pb_section_1  et_pb_css_mix_blend_mode et_section_regular et_block_section\" >\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_d4_element et_pb_row et_pb_row_1  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_1  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_with_border et_pb_module et_d4_element et_pb_text et_pb_text_1  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\">DYES<\/h2><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_2  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_2  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_2  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h3>Cell Excluded Dyes<\/h3><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_3  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_1_2 et_pb_column et_pb_column_3  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_3  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><p><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/SurfaceOnly.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/SurfaceOnly-300x300.png\" width=\"300\" height=\"300\" alt=\"MG b Tau\" class=\"wp-image-31 alignnone size-medium\" style=\"display: block; margin-left: auto; margin-right: auto;\" srcset=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/SurfaceOnly-300x300.png 300w, https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/SurfaceOnly-150x150.png 150w, https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/SurfaceOnly.png 459w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<h3 style=\"text-align: center;\">MG-b-tau<\/h3>\n<p style=\"text-align: center;\">preferred cell-excluded dye<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_2 et_pb_column et_pb_column_4  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_4  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><p><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/SurfaceOnly.png\"><\/a><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/hcm-dyedron2.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/hcm-dyedron2-300x149.jpg\" width=\"300\" height=\"149\" alt=\"HCM Dyedron multi-donor arrays with a single fluorogen acceptor\" class=\"wp-image-32 alignnone size-medium\" style=\"display: block; margin-left: auto; margin-right: auto;\" \/><\/a><\/p>\n<h3 style=\"text-align: center;\">HCM-dyedron<\/h3>\n<p style=\"text-align: center;\">multi-donor arrays with a single fluorogen acceptor<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_4  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_5  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_5  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h3>Cell Permeant Dyes<\/h3><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_5  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_1_3 et_pb_column et_pb_column_6  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_6  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><p><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/mg-ester_tubulin_c64017.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/mg-ester_tubulin_c64017-300x300.jpg\" width=\"300\" height=\"300\" alt=\"mg-ester preferred cell permeant dye\" class=\"wp-image-34 alignnone size-medium\" style=\"display: block; margin-left: auto; margin-right: auto;\" \/><\/a><\/p>\n<h3 style=\"text-align: center;\">MG-ester<\/h3>\n<p style=\"text-align: center;\">preferred cell-permeant dye<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_3 et_pb_column et_pb_column_7  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_7  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><p><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/dL5-BK-MHN-3m.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/dL5-BK-MHN-3m-300x300.jpg\" width=\"300\" height=\"300\" alt=\"Green cell, permeant dye\" class=\"wp-image-36 alignnone size-medium\" style=\"display: block; margin-left: auto; margin-right: auto;\" \/><\/a><\/p>\n<h3 style=\"text-align: center;\">mhn-ester<\/h3>\n<p style=\"text-align: center;\">green cell-permeant dye<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_3 et_pb_column et_pb_column_8  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_8  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><p><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/blur2.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/blur2-300x149.jpg\" width=\"300\" height=\"149\" alt=\"Blur 2 FET cell permeant dye\" class=\"wp-image-33 alignnone size-medium\" style=\"display: block; margin-left: auto; margin-right: auto;\" \/><\/a><\/p>\n<h3 style=\"text-align: center;\">blur2<\/h3>\n<p style=\"text-align: center;\">FRET cell-permeant dye<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_6  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_1_2 et_pb_column et_pb_column_9  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_9  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h3>Photosensitizer Dyes<\/h3><\/div>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_10  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><p><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/mg2i.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/mg2i.jpg\" width=\"300\" height=\"300\" alt=\"mg-2i produces singlet oxygen\" class=\"wp-image-35 alignnone size-full\" style=\"display: block; margin-left: auto; margin-right: auto;\" srcset=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/mg2i.jpg 176w, https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/mg2i-150x150.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<h3 style=\"text-align: center;\">mg-2i<\/h3>\n<p style=\"text-align: center;\">produces Singlet Oxygen<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_2 et_pb_column et_pb_column_10  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_11  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h3>Sensor Dyes<\/h3><\/div>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_12  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><p><a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/Amine-B2AR-phSensor_7_MMStack_Pos1.ome_.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/Amine-B2AR-phSensor_7_MMStack_Pos1.ome_-300x300.jpg\" width=\"300\" height=\"300\" alt=\"Amine-B2AR-phSensor_7\" class=\"wp-image-37 alignnone size-medium\" style=\"display: block; margin-left: auto; margin-right: auto;\" \/><\/a><\/p>\n<h3 style=\"text-align: center;\">cy3pH-MG<\/h3>\n<p style=\"text-align: center;\">detects changes in pH<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_section et_pb_section_2 et_pb_with_background  et_pb_css_mix_blend_mode et_section_regular et_block_section\" >\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_d4_element et_pb_row et_pb_row_7  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_11  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_with_border et_pb_module et_d4_element et_pb_text et_pb_text_13  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\">Protocol List<\/h2><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_8  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_12  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_14  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h3>Protocol List<\/h3><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_9  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_1_4 et_pb_column et_pb_column_13  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_icon et_pb_icon_0\">\n\t\t\t\t<span class=\"et_pb_icon_wrap \"><span class=\"et-pb-icon\">\uf492<\/span><\/span>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_15  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h4>Suspension and Handling<\/h4>\n<p>How to properly suspend dyes and determine their concentration <a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/Protocol-SuspensionConcentrationStorage-2.pdf\" rel=\"attachment wp-att-193\">Protocol-Suspension Concentration Storage<\/a><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_4 et_pb_column et_pb_column_14  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_icon et_pb_icon_1\">\n\t\t\t\t<span class=\"et_pb_icon_wrap \"><span class=\"et-pb-icon\">\uf111<\/span><\/span>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_16  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h4>Surface Labeling FAP-Fusions<\/h4>\n<p>Surface selective labeling is achieved by adding a cell-excluded dye to living cells in a physiological buffer. Labeling is essentially instantaneous, if dye is added at mid-nanomolar concentrations. <a href=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/Protocol-SurfaceLabeling.pdf\" rel=\"attachment wp-att-192\">Protocol-SurfaceLabeling<\/a>.<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_4 et_pb_column et_pb_column_15  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_icon et_pb_icon_2\">\n\t\t\t\t<span class=\"et_pb_icon_wrap \"><span class=\"et-pb-icon\">\uf192<\/span><\/span>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_17  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h4>Intraceullar labeling fap-fusions<\/h4>\n<p>Total protein pool labeling is achieved by adding a cell-permeant dye to living cells in a physiological buffer. After equilibration for a short time, cell-permeant dye penetrates the cell to label intracellular expressed FAP sites. Labeling is rapid (10 min), and dye does not need to be washed out to achieve selective labeling.<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_4 et_pb_column et_pb_column_16  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_icon et_pb_icon_3\">\n\t\t\t\t<span class=\"et_pb_icon_wrap \"><span class=\"et-pb-icon\">\uf192<\/span><\/span>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_18  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h4>Surface and Intracellular dye labeling combinations<\/h4>\n<p>Label distinctive pools of protein in different colors.<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_section et_pb_section_3  et_pb_css_mix_blend_mode et_section_regular et_block_section\" >\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_d4_element et_pb_row et_pb_row_10  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_17  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_with_border et_pb_module et_d4_element et_pb_text et_pb_text_19  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\">Vectors and Plasmid Maps<\/h2><\/div>\n\t\t\t<\/div><div class=\"et_pb_button_module_wrapper et_pb_button_0_wrapper et_pb_button_alignment_center et_pb_module \">\n\t\t\t\t<a class=\"et_pb_button et_d4_element et_pb_button_0 et_pb_bg_layout_light et_block_module\" href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" data-icon=\"E\">Click for Bruchez Lab Addgene Link<\/a>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_11  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_18  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_20  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h3>Vectors<\/h3><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_12  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_1_3 et_pb_column et_pb_column_19  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_image et_pb_image_0\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<span class=\"et_pb_image_wrap \"><img loading=\"lazy\" decoding=\"async\" width=\"397\" height=\"413\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_mamalia.png\" alt=\"Mammalian Expression\" title=\"Mammalian Expression\" srcset=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_mamalia.png 397w, https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_mamalia-288x300.png 288w\" sizes=\"(max-width: 397px) 100vw, 397px\" class=\"wp-image-9\" \/><\/span>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_21  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h4>Mammalian Expression<\/h4>\n<h5>Organelle Targeted FAPs<\/h5>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-COX4-COX8-dL5-2XG4S-mCer3<\/a><br \/>\n(Mitochondria Addgene 73208)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-kappa-myc-dL5-2XG4S-mCer-KDEL<\/a><br \/>\n(Endoplasmic Reticulum Addgene 73209)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-KozATG-dL5-2XG4S-mCer3<\/a><br \/>\n(Cytosolic Addgene 73207)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-myc-dL5-2XG4S-mCerSKL<\/a><br \/>\n(Peroxisomal Addgene 73210)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-NLS-myc-dL5-2XG4S-mCer3<\/a><br \/>\n(Nuclear Addgene 73205)<\/p>\n<h5>Actin-FAPs<\/h5>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-KozATG-dH6.2-2XG4S-actin<\/a><br \/>\nAddgene 73263<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-KozATG-dL5-2XG4S-actin<\/a><br \/>\nAddgene 73262<\/p>\n<h5>Membrane Proteins<\/h5>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-kappa-dL5-2XG4S-TMst<\/a><br \/>\n(PDGFR TM anchored surface-expression FAP Addgene 73206)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-kappa-HA-dL5-BKa (KCNMA1)<\/a><br \/>\n(KCNMA1)<\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_3 et_pb_column et_pb_column_20  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_image et_pb_image_1\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<span class=\"et_pb_image_wrap \"><img loading=\"lazy\" decoding=\"async\" width=\"392\" height=\"402\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_bacterial-expressions.png\" alt=\"Bacterial Expression\" title=\"Bacterial Expression\" srcset=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_bacterial-expressions.png 392w, https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_bacterial-expressions-293x300.png 293w\" sizes=\"(max-width: 392px) 100vw, 392px\" class=\"wp-image-8\" \/><\/span>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_22  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h4>Bacterial Expression<\/h4>\n<h5>FAP dL5 Bacterial Expression<\/h5>\n<p>(Generally, we express from oxidizing strains such as Rosetta B-Gami2)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pET21a-10XHis-GST-HRV-dL5(YW)<\/a><br \/>(Addgene 74214)<\/p>\n<h5>Affibodies and Affi-FAP Constructs<\/h5>\n<p>(See Wang, Y et al, Bioconj. Chem. 2015)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pET21a-10XHis-GST-HRV-dHer1cysteineST<\/a><br \/>(double Her1-affibody Z1907 with cysteine at C-terminus Addgene 73218)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pET21a-10XHis-GST-HRV-dL5-Her1<\/a><br \/>(FAP-Affibody construct, Her1 Z1907 affibeody Addgene 73215)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pET21a-10XHis-GST-HRV-Her1-dL5-Her1<\/a><br \/>(Affibody-FAP-Affibody construct, Her1 affibeody Z1907 Addgene 73217)<\/p>\n<p><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pET21a-10XHis-GST-HRV-Her1-dL5<\/a><br \/>(Affibody-FAP construct, Her1 affibody Z1907 Addgene 73216)<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_1_3 et_pb_column et_pb_column_21  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_image et_pb_image_2\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<span class=\"et_pb_image_wrap \"><img loading=\"lazy\" decoding=\"async\" width=\"396\" height=\"409\" src=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_yeast-expressions.png\" alt=\"icon for yeast\" title=\"icon for yeast\" srcset=\"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_yeast-expressions.png 396w, https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-content\/uploads\/sites\/35\/2023\/11\/icons_yeast-expressions-290x300.png 290w\" sizes=\"(max-width: 396px) 100vw, 396px\" class=\"wp-image-27\" \/><\/span>\n\t\t\t<\/div><div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_23  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h4>Yeast Expression<\/h4><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_row et_pb_row_13  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_4_4 et_pb_column et_pb_column_22  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_24  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h3 style=\"text-align: center;\">Plasmid Maps<\/h3>\n<p style=\"text-align: center;\">Transmembrane FAP<br \/><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-kappa-myc-dL5-2XG4S-TMst<\/a><br \/><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-kappa-dL5-2XG4S-TMst<\/a><\/p>\n<p style=\"text-align: center;\">Big Potassium Conductance Channel (BKa)<br \/><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">dL5-BKa pcDNA<\/a><\/p>\n<p style=\"text-align: center;\">Nuclear Localized FAP-mCer3 Fusion Protein<br \/><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3-NLS-myc-dL5-2XG4S-mCer<\/a><\/p>\n<p style=\"text-align: center;\">Mitochondrial Localized FAP-mCer3 Fusion Protein<br \/><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-COX4-COX8-dL5-2XG4S-mCer<\/a><\/p>\n<p style=\"text-align: center;\">ER-Localized FAP-mCer3 Fusion Protein<br \/><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-kappa-myc-dL5-2XG4S-mCer-KDEL<\/a><\/p>\n<p style=\"text-align: center;\">Cytoplasmic Localized FAP-Mcer3 Fusion Protein \u2013penetrates nucleus<br \/><a href=\"https:\/\/www.addgene.org\/Marcel_Bruchez\/\" target=\"_blank\" rel=\"noopener\">pcDNA3.1-KozATG-dL5-2XG4S-mCer<\/a><\/p>\n<p style=\"text-align: center;\">________<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/biologylabs.utah.edu\/jorgensen\/wayned\/ape\/\" target=\"_blank\" rel=\"noopener\">ApE Plasmid Editor<\/a><br \/>by M. Wayne Davis<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><div class=\"et_d4_element et_pb_section et_pb_section_4 et_pb_with_background  et_pb_css_mix_blend_mode et_section_regular et_block_section\" >\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_d4_element et_pb_row et_pb_row_14  et_pb_css_mix_blend_mode et_block_row\">\n\t\t\t\t<div class=\"et_d4_element et_pb_column_2_5 et_pb_column et_pb_column_23  et_pb_css_mix_blend_mode et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_icon et_pb_icon_4\">\n\t\t\t\t<span class=\"et_pb_icon_wrap \"><span class=\"et-pb-icon\">\uf0e0<\/span><\/span>\n\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_d4_element et_pb_column_3_5 et_pb_column et_pb_column_24  et_pb_css_mix_blend_mode et-last-child et_block_column\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module et_d4_element et_pb_text et_pb_text_25  et_pb_text_align_left et_pb_bg_layout_light\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_text_inner\"><h2>Request for Materials<\/h2>\n<p>Carnegie Mellon University<br \/>Office: MI-749, Lab: MI-755<br \/>4400 Fifth Ave.<br \/>Pittsburgh, PA 15213<br \/>Phone: (412) 268-9661<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":10,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-125","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/pages\/125","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/comments?post=125"}],"version-history":[{"count":31,"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/pages\/125\/revisions"}],"predecessor-version":[{"id":195,"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/pages\/125\/revisions\/195"}],"wp:attachment":[{"href":"https:\/\/labs.bio.cmu.edu\/bruchez-lab\/wp-json\/wp\/v2\/media?parent=125"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}