![\"\"](\"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-content\/uploads\/sites\/2\/2025\/03\/cropped-logo5.gif\" "\\"cropped-logo5.gif\\""){kind=logo}
<\/span>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>Translation Regulation in Fungal Pathogens<\/h1><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t![\"\"](\"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-content\/uploads\/sites\/2\/2025\/03\/candida.gif\" "\\"candida\\"")
<\/span>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\tCandida albicans<\/em> is an opportunistic infectious yeast. Pathogenicity requires detecting and responding to stress in divergent physiological microenvironments. Under stress, C. albicans<\/em> undergoes the process of filamentation, shifting from commensal yeast to virulent hyphae. In addition, C albicans<\/em> can also form biofilms. Under different hyphal-inducing conditions C. albicans<\/em> activates distinct transcriptional programs. While transcriptional control of hyphal growth has been extensively studied, comparatively little is known about post-transcriptional regulation of this significant morphological shift. We use CRISPR-Cas9, mRNA seq, and ribosome profiling to examine the role that post-transcriptional translation regulation and P-body formation plays in the yeast to hyphae transition. Recently we have found that in lab and clinical strains of C. albicans<\/em>, mRNA decay factors play distinct roles in regulating cell morphology and that Dhh1 contributes to environmentally appropriate expression of the stress response and hyphal growth. Our work supports distinct requirements for specific mRNA decay factors, bolstering evidence for post-transcriptional regulation of filamentation in C. albicans<\/em>. We are also applying these methods to study translation regulation in another pathogenic fungus, Cryptococcus neoformans<\/em>.<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\tPhotographs by Melissa Tosiano<\/em><\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\tSelected Publications<\/h3><\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\tTosiano MA, Lanni F, Mitchell AP, McManus CJ. (2025) Roles of P-body factors in Candida albicans filamentation and stress response. PLOS Genetics<\/em><\/a><\/p>\nCravener MV, Do E, May G, Zarnowski R, Andes DR, McManus CJ<\/strong>, Mitchell AP. (2023) Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network. PLoS Pathogens<\/a><\/em><\/p>\nDo E, Cravener MV, Huang MY, May G, McManus CJ<\/strong>, Mitchell AP. (2022) Collaboration between Antagonistic Cell Type Regulators Governs Natural Variation in the Candida albicans<\/em> Biofilm and Hyphal Gene Expression Network. mBio<\/em><\/a><\/p>\nLagree K, Woolford CA, Huang MY, May G, McManus CJ<\/strong>, Solis NV, Filler SG, Mitchell AP. (2020) Roles of Candida albicans<\/em> Mig1 and Mig2 in glucose repression, pathogenicity traits, and SNF1 essentiality. PLoS Genetics<\/a><\/em><\/p>\nHuang MY, Woolford CA, May G, McManus CJ, Mitchell AP. (2019) Circuit diversification in a biofilm regulatory network. PLoS Pathogens<\/em><\/a><\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":21,"featured_media":0,"parent":8,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"[caption id=\"attachment_469\" align=\"alignleft\" width=\"272\"]![\"\"](\"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-content\/uploads\/sites\/2\/2025\/03\/candida-272x300.gif\")
Photographs by Melissa Tosiano[\/caption]\r\n\r\nCandia albicans<\/em> is an opportunistic infectious yeast. Pathogenicity requires detecting and responding to stress in divergent physiological microenvironments. Under stress, C. albicans<\/em> undergoes the process of filamentation, shifting from commensal yeast to virulent hyphae. In addition, C albicans<\/em> can also form biofilms. Under different hyphal-inducing conditions C. albicans<\/em> activates distinct transcriptional programs. While transcriptional control of hyphal growth has been extensively studied, comparatively little is known about post-transcriptional regulation of this significant morphological shift. We use CRISPR-Cas9, mRNA seq, and ribosome profiling to examine the role that post-transcriptional translation regulation and P-body formation plays in the yeast to hyphae transition. Recently we have found that in lab and clinical strains of C. albicans<\/em>, mRNA decay factors play distinct roles in regulating cell morphology and that Dhh1 contributes to environmentally appropriate expression of the stress response and hyphal growth. Our work supports distinct requirements for specific mRNA decay factors, bolstering evidence for post-transcriptional regulation of filamentation in C. albicans<\/em>. We are also applying these methods to study translation regulation in another pathogenic fungus, Cryptococcus neoformans<\/em>.\r\n\r\n \r\nSelected Publications<\/h3>\r\nTosiano MA, Lanni F, Mitchell AP, McManus CJ. (2025) Roles of P-body factors in Candida albicans filamentation and stress response. Accepted to PLOS Genetics<\/em><\/a>\r\n\r\nCravener MV, Do E, May G, Zarnowski R, Andes DR, McManus CJ<\/strong>, Mitchell AP. (2023) Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network. PLoS Pathogens<\/a><\/em>\r\n\r\nDo E, Cravener MV, Huang MY, May G, McManus CJ<\/strong>, Mitchell AP. (2022) Collaboration between Antagonistic Cell Type Regulators Governs Natural Variation in the Candida albicans<\/em> Biofilm and Hyphal Gene Expression Network. mBio<\/em><\/a>\r\n\r\nLagree K, Woolford CA, Huang MY, May G, McManus CJ<\/strong>, Solis NV, Filler SG, Mitchell AP. (2020) Roles of Candida albicans<\/em> Mig1 and Mig2 in glucose repression, pathogenicity traits, and SNF1 essentiality. PLoS Genetics<\/a><\/em>\r\n\r\nHuang MY, Woolford CA, May G, McManus CJ, Mitchell AP. (2019) Circuit diversification in a biofilm regulatory network. PLoS Pathogens<\/em><\/a>\r\n\r\n \r\n\r\n ","_et_gb_content_width":"","footnotes":""},"class_list":["post-329","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/ParKnc-5j","_links":{"self":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/users\/21"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/comments?post=329"}],"version-history":[{"count":19,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/329\/revisions"}],"predecessor-version":[{"id":711,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/329\/revisions\/711"}],"up":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/8"}],"wp:attachment":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/media?parent=329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/span>\n\t\t\t<\/div>\n\t\t\t<\/div>Photographs by Melissa Tosiano<\/em><\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Tosiano MA, Lanni F, Mitchell AP, McManus CJ. (2025) Roles of P-body factors in Candida albicans filamentation and stress response. PLOS Genetics<\/em><\/a><\/p>\n Cravener MV, Do E, May G, Zarnowski R, Andes DR, McManus CJ<\/strong>, Mitchell AP. (2023) Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network. PLoS Pathogens<\/a><\/em><\/p>\n Do E, Cravener MV, Huang MY, May G, McManus CJ<\/strong>, Mitchell AP. (2022) Collaboration between Antagonistic Cell Type Regulators Governs Natural Variation in the Candida albicans<\/em> Biofilm and Hyphal Gene Expression Network. mBio<\/em><\/a><\/p>\n Lagree K, Woolford CA, Huang MY, May G, McManus CJ<\/strong>, Solis NV, Filler SG, Mitchell AP. (2020) Roles of Candida albicans<\/em> Mig1 and Mig2 in glucose repression, pathogenicity traits, and SNF1 essentiality. PLoS Genetics<\/a><\/em><\/p>\n Huang MY, Woolford CA, May G, McManus CJ, Mitchell AP. (2019) Circuit diversification in a biofilm regulatory network. PLoS Pathogens<\/em><\/a><\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":21,"featured_media":0,"parent":8,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"[caption id=\"attachment_469\" align=\"alignleft\" width=\"272\"]Selected Publications<\/h3><\/div>\n\t\t\t<\/div>
Photographs by Melissa Tosiano[\/caption]\r\n\r\nCandia albicans<\/em> is an opportunistic infectious yeast. Pathogenicity requires detecting and responding to stress in divergent physiological microenvironments. Under stress, C. albicans<\/em> undergoes the process of filamentation, shifting from commensal yeast to virulent hyphae. In addition, C albicans<\/em> can also form biofilms. Under different hyphal-inducing conditions C. albicans<\/em> activates distinct transcriptional programs. While transcriptional control of hyphal growth has been extensively studied, comparatively little is known about post-transcriptional regulation of this significant morphological shift. We use CRISPR-Cas9, mRNA seq, and ribosome profiling to examine the role that post-transcriptional translation regulation and P-body formation plays in the yeast to hyphae transition. Recently we have found that in lab and clinical strains of C. albicans<\/em>, mRNA decay factors play distinct roles in regulating cell morphology and that Dhh1 contributes to environmentally appropriate expression of the stress response and hyphal growth. Our work supports distinct requirements for specific mRNA decay factors, bolstering evidence for post-transcriptional regulation of filamentation in C. albicans<\/em>. We are also applying these methods to study translation regulation in another pathogenic fungus, Cryptococcus neoformans<\/em>.\r\n\r\n \r\nSelected Publications<\/h3>\r\nTosiano MA, Lanni F, Mitchell AP, McManus CJ. (2025) Roles of P-body factors in Candida albicans filamentation and stress response. Accepted to PLOS Genetics<\/em><\/a>\r\n\r\nCravener MV, Do E, May G, Zarnowski R, Andes DR, McManus CJ<\/strong>, Mitchell AP. (2023) Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network. PLoS Pathogens<\/a><\/em>\r\n\r\nDo E, Cravener MV, Huang MY, May G, McManus CJ<\/strong>, Mitchell AP. (2022) Collaboration between Antagonistic Cell Type Regulators Governs Natural Variation in the Candida albicans<\/em> Biofilm and Hyphal Gene Expression Network. mBio<\/em><\/a>\r\n\r\nLagree K, Woolford CA, Huang MY, May G, McManus CJ<\/strong>, Solis NV, Filler SG, Mitchell AP. (2020) Roles of Candida albicans<\/em> Mig1 and Mig2 in glucose repression, pathogenicity traits, and SNF1 essentiality. PLoS Genetics<\/a><\/em>\r\n\r\nHuang MY, Woolford CA, May G, McManus CJ, Mitchell AP. (2019) Circuit diversification in a biofilm regulatory network. PLoS Pathogens<\/em><\/a>\r\n\r\n \r\n\r\n ","_et_gb_content_width":"","footnotes":""},"class_list":["post-329","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/ParKnc-5j","_links":{"self":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/users\/21"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/comments?post=329"}],"version-history":[{"count":19,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/329\/revisions"}],"predecessor-version":[{"id":711,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/329\/revisions\/711"}],"up":[{"embeddable":true,"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/pages\/8"}],"wp:attachment":[{"href":"https:\/\/labs.bio.cmu.edu\/mcmanus\/wp-json\/wp\/v2\/media?parent=329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}