Equipment
Microscopes
Please contact Aleksandra Klimas for access and training schedule microscopes through CABIS’ Skedda.
Microscopes: Confocals
Zeiss 880 Laser Scanning
This microscope system provides one-photon (1-p) confocal, photo-manipulation and spectral unmixing fluorescence technologies.
Six laser lines (405, 458, 488, 514, 561 and 633 nm). Image acquisition includes multi-color, z-stack, time lapse and multi-position with hardware autofocusing, which is suitable for applications such as localization, colocalization, time-lapse, uncaging, FRET, FRAP, and ratio imaging. The instrument also has fluorescence correlation spectroscopy capabilities.
Pricing:
Peak (Mon-Fri, 9AM-5PM): $32/hr
Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr
Andor Revolution XD Spinning Disk Microscope
This system provides a range of laser-illuminated microscopy solutions, including spinning disk confocal, and total internal reflection fluorescence (TIRF) modalities, ideal for live-cell imaging and photo-manipulation. There are four laser lines (405, 488, 560, and 640 nm) and three cameras (two EMCCD and one CCD).
Pricing:
Peak (Mon-Fri, 9AM-5PM): $32/hr
Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr
Microscopes: Widefields
Zeiss AxioObserver Z1 Wide-field LED-based inverted epifl. microscope
Axio Observer is your stable research stand for demanding multimodal imaging of living and fixed specimens. This inverted microscope offers the latest generation of LED illumination for gentle imaging. And creates the optimal environment for a whole range of samples to deliver reliable, reproducible data.
Pricing:
Peak (Mon-Fri, 9AM-5PM): $32/hr
Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr
Nikon TiE WideField LED-based inverted epifluorescence HCS/ area imager
Combined with NIS-Elements imaging software, the Ti supports diverse image acquisition and analysis methods such as multi-dimensional time-lapse imaging to acquire temporal, spatial and spectral information of fast, dynamic live cell processes. Intelligently designed automation and further expansion of Nikon’s powerful modular approach make the Ti ideal for applications such as confocal, FRET, High Content Analysis (HCS), and photobleaching/photo activation to study interaction of fluorescence protein molecules in living cells and tissues. Nikon’s exclusive Perfect Focus System (PFS) is now incorporated into the nosepiece unit and allows for the simultaneous use of two separate levels for additional illuminators or detectors. The newly developed “full intensity” phase contrast unit enables acquisition of incredible phase contrast images without the use of light-attenuating phase contrast objectives
Pricing:
Peak (Mon-Fri, 9AM-5PM): $32/hr
Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr
Flow Cytometers
Please contact Aleksandra Klimas for access and training schedule flow cytometers through CABIS’ ClusterMarket.
Intellicyt / Accuri High Throughput Flow Cytometer
The Intellicyt front end allows rapid sampling of 96-well plates and feed of these samples to the attached Accuri C6 flow analyzer to produce high-throughput single cell analysis data of up to two fluorescence parameters. The Accuri is equipped with two excitation lasers, 488 and 640 nm.
Pricing:
Peak (Mon-Fri, 9AM-5PM): $36/hr
Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $27/hr
Plate reader
Tecan Spark
Pricing:
Peak (Mon-Fri, 9AM-5PM): $28/hr
Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $21/hr
Cryomicrotome
Shandon Cryomicrotome
Pricing:
Free to use
UV VIS
Agilent Cary 60 UV VIS
Pricing:
Free to use
Image Analysis Software
IMARIS PRO
Pricing:
Peak (Mon-Fri, 9AM-5PM): $24/hr
Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $18/hr
Illumination Boxes
LED Light Box (365, 395,440,525, 660)
660 Laser Light Box
660 LED Light Box
Pricing:
Free to use
Scheduling Link for equipment
Log into Skedda. To be added to the ClusterMarket CABIS Calendar please contact the imaging director, Aleksandra Klimas.