Equipment

Microscopes

Please contact Aleksandra Klimas for access and training schedule microscopes through CABIS’ Skedda.

Microscopes: Confocals

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Zeiss 880 Laser Scanning

This microscope system provides one-photon (1-p) confocal, photo-manipulation and spectral unmixing fluorescence technologies.

Six laser lines (405, 458, 488, 514, 561 and 633 nm). Image acquisition includes multi-color, z-stack, time lapse and multi-position with hardware autofocusing, which is suitable for applications such as localization, colocalization, time-lapse, uncaging, FRET, FRAP, and ratio imaging.
The instrument also has fluorescence correlation spectroscopy capabilities.

 

Pricing: 

Peak (Mon-Fri, 9AM-5PM): $32/hr

Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr

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Andor Revolution XD Spinning Disk Microscope

This system provides a range of laser-illuminated microscopy solutions, including spinning disk confocal, and total internal reflection fluorescence (TIRF)  modalities, ideal for live-cell imaging and photo-manipulation. There are four laser lines (405, 488, 560, and 640 nm) and three cameras (two EMCCD and one CCD).

 

Pricing: 

Peak (Mon-Fri, 9AM-5PM): $32/hr

Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr

Microscopes: Widefields 

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Zeiss AxioObserver Z1 Wide-field LED-based inverted epifl. microscope

Axio Observer is your stable research stand for demanding multimodal imaging of living and fixed specimens. This inverted microscope offers the latest generation of LED illumination for gentle imaging. And creates the optimal environment for a whole range of samples to deliver reliable, reproducible data.

 

Pricing: 

Peak (Mon-Fri, 9AM-5PM): $32/hr

Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr

nikon_tie_widefield

Nikon TiE WideField LED-based inverted epifluorescence HCS/ area imager

Combined with NIS-Elements imaging software, the Ti supports diverse image acquisition and analysis methods such as multi-dimensional time-lapse imaging to acquire temporal, spatial and spectral information of fast, dynamic live cell processes. Intelligently designed automation and further expansion of Nikon’s powerful modular approach make the Ti ideal for applications such as confocal, FRET, High Content Analysis (HCS), and photobleaching/photo activation to study interaction of fluorescence protein molecules in living cells and tissues. Nikon’s exclusive Perfect Focus System (PFS) is now incorporated into the nosepiece unit and allows for the simultaneous use of two separate levels for additional illuminators or detectors. The newly developed “full intensity” phase contrast unit enables acquisition of incredible phase contrast images without the use of light-attenuating phase contrast objectives

 

Pricing: 

Peak (Mon-Fri, 9AM-5PM): $32/hr

Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $28/hr

Flow Cytometers


Please contact Aleksandra Klimas for access and training schedule flow cytometers through CABIS’ ClusterMarket.

Intellicyt / Accuri High Throughput Flow Cytometer

The Intellicyt front end allows rapid sampling of 96-well plates and feed of these samples to the attached Accuri C6 flow analyzer to produce high-throughput single cell analysis data of up to two fluorescence parameters. The Accuri is equipped with two excitation lasers, 488 and 640 nm.

Pricing:

Peak (Mon-Fri, 9AM-5PM): $36/hr

Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $27/hr

Plate reader


Tecan Spark

 

Pricing:

Peak (Mon-Fri, 9AM-5PM): $28/hr

Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $21/hr

Cryomicrotome


Shandon Cryomicrotome

 

Pricing:

Free to use

UV VIS


Agilent Cary 60 UV VIS

 

Pricing:

Free to use

Image Analysis Software


 IMARIS PRO

Pricing:

Peak (Mon-Fri, 9AM-5PM): $24/hr

Off Peak: (Mon-Fri, 5PM-9AM; Weekends All Day): $18/hr

Illumination Boxes


LED Light Box (365, 395,440,525, 660)

660 Laser Light Box

660 LED Light Box

 

Pricing:

Free to use

Scheduling Link for equipment


Log into Skedda. To be added to the ClusterMarket CABIS Calendar please contact the imaging director, Aleksandra Klimas.