Gene expression varies between individuals and species, and this variation is largely responsible for phenotypic diversity and disease. Research in the McManus lab focuses on understanding the genetic causes of variation in gene expression. Gene expression involves transcription of DNA into mRNA, alternative splicing of mRNA, translation of mRNA into proteins, and regulation of mRNA and protein levels through turnover pathways. Differences in the regulatory networks controlling these processes lead to gene expression variation. Our lab uses high-throughput sequencing and bioinformatics to investigate the roles of upstream open reading frames (uORFs) and RNA structure in the regulation of gene expression.